{"title":"Accessories \u0026 Bacteriostatic Water","description":"\u003cp\u003eBacteriostatic water and the supplies needed to reconstitute lyophilised research peptides. Laboratory use only.\u003c\/p\u003e","products":[{"product_id":"bacteriostatic-water","title":"Bacteriostatic Water","description":"\u003cstyle\u003e.pp-pdp{--nv:#0d2233;--tl:#3a9ab5;--ink:#1f2937;--mut:#5b6b7a;--ln:#e3e8ee;--sf:#f5f8fa;--gr:#1E9E5A;font-family:Montserrat,-apple-system,sans-serif;color:var(--ink);line-height:1.6;margin:34px 0 0;width:100%;max-width:100%;overflow-x:hidden;background:#fff;text-align:left}.pp-pdp *{box-sizing:border-box}.pp-pdp h2,.pp-pdp h3{color:var(--nv);font-weight:800;line-height:1.15;margin:0 0 14px}.pp-pdp p{color:var(--ink);margin:0 0 14px}.pp-pdp p:last-child{margin-bottom:0}.pp-pdp a{color:#0d6c8a}.pp-pdp .pp-in{max-width:1180px;margin:0 auto;padding:0 24px}.pp-pdp .pp-kick{color:var(--tl);font-size:12px;letter-spacing:2.16px;text-transform:uppercase;font-weight:600;margin:0 0 12px}.pp-pdp .pp-sec{padding:56px 0}.pp-pdp .pp-alt{background:var(--sf)}.pp-pdp .pp-sec h2{font-size:30px}.pp-pdp .pp-prose p{font-size:15.5px;color:var(--mut)}.pp-pdp .pp-prose p strong{color:var(--nv)}.pp-pdp .pp-wide{max-width:900px}.pp-pdp .pp-g2{display:grid;grid-template-columns:1.05fr .95fr;gap:48px;align-items:start}.pp-pdp .pp-media{position:sticky;top:24px}.pp-pdp .pp-img{width:100%;height:auto;display:block;border-radius:14px;border:1px solid var(--ln);background:#fff}.pp-pdp .pp-fig{margin:30px 0 0;max-width:900px}.pp-pdp .pp-facts{background:var(--nv);border-top:4px solid var(--tl);padding:28px 0}.pp-pdp .pp-facts .pp-in{display:grid;grid-template-columns:repeat(auto-fit,minmax(180px,1fr));gap:22px}.pp-pdp .pp-fi{text-align:center;padding:4px 14px;border-left:1px solid rgba(255,255,255,.14)}.pp-pdp .pp-fi:first-child{border-left:0}.pp-pdp .pp-fi b{display:block;color:#fff;font-weight:800;line-height:1.2;margin-bottom:8px}.pp-pdp .pp-fi .pp-v1{font-size:27px}.pp-pdp .pp-fi .pp-v2{font-size:17px}.pp-pdp .pp-fi .pp-v3{font-size:13.5px;font-weight:700;line-height:1.45}.pp-pdp .pp-fi span{display:block;color:var(--tl);font-size:11.5px;letter-spacing:1.4px;text-transform:uppercase;font-weight:700}.pp-pdp .pp-cards{display:grid;grid-template-columns:repeat(auto-fit,minmax(230px,1fr));gap:20px}.pp-pdp .pp-card{background:#fff;border:1px solid var(--ln);border-radius:12px;padding:24px;box-shadow:0 2px 10px rgba(13,34,51,.05)}.pp-pdp .pp-card .pp-num{display:block;font-size:12px;letter-spacing:1.4px;font-weight:800;color:var(--tl);margin-bottom:10px}.pp-pdp .pp-card p{font-size:14.5px;color:var(--mut);margin:0}.pp-pdp .pp-faq{max-width:880px}.pp-pdp .pp-ac{background:#fff;border:1px solid var(--ln);border-radius:10px;margin-bottom:12px;overflow:hidden}.pp-pdp .pp-ac summary{list-style:none;cursor:pointer;padding:18px 54px 18px 22px;position:relative;font-weight:700;font-size:16.5px;color:var(--nv)}.pp-pdp .pp-ac summary::-webkit-details-marker{display:none}.pp-pdp .pp-ac summary:after{content:\"\";position:absolute;right:24px;top:50%;width:9px;height:9px;border-right:2px solid var(--tl);border-bottom:2px solid var(--tl);transform:translateY(-70%) rotate(45deg);transition:transform .18s}.pp-pdp .pp-ac[open] summary:after{transform:translateY(-20%) rotate(-135deg)}.pp-pdp .pp-ac summary:hover{background:var(--sf)}.pp-pdp .pp-ac .pp-ab{padding:0 22px 20px;color:var(--mut);font-size:15px}.pp-pdp .pp-ac .pp-ab p{color:var(--mut);font-size:15px}.pp-pdp .pp-links{background:#fff;border-top:1px solid var(--ln);padding:34px 0 44px}.pp-pdp .pp-chips{display:flex;flex-wrap:wrap;gap:10px}.pp-pdp .pp-chip{display:inline-block;padding:10px 16px;border:1px solid var(--ln);border-radius:6px;background:#fff;color:var(--nv)!important;font-size:13.5px;font-weight:600;text-decoration:none;line-height:1.3}.pp-pdp .pp-chip:hover{border-color:var(--tl)}.pp-pdp .pp-chip-lead{background:var(--nv);color:#fff!important;border-color:var(--nv)}.pp-pdp .pp-chip-alt{background:var(--sf)} @media(max-width:900px){.pp-pdp .pp-g2{grid-template-columns:1fr;gap:28px}.pp-pdp .pp-media{position:static}.pp-pdp .pp-sec{padding:40px 0}.pp-pdp .pp-sec h2{font-size:24px}.pp-pdp .pp-in{padding:0 18px}.pp-pdp .pp-facts .pp-in{grid-template-columns:repeat(2,1fr);gap:18px}.pp-pdp .pp-fi{border-left:0;text-align:left;padding:0}.pp-pdp .pp-fi .pp-v1{font-size:22px}.pp-pdp .pp-cards{grid-template-columns:1fr} } @media(max-width:520px){.pp-pdp .pp-facts .pp-in{grid-template-columns:1fr}}\u003c\/style\u003e\n\u003cdiv class=\"pp-pdp\"\u003e\n\u003csection class=\"pp-facts\" id=\"specs\"\u003e\u003cdiv class=\"pp-in\"\u003e\n\u003cdiv class=\"pp-fi\"\u003e\n\u003cb class=\"pp-v3\"\u003eWater for injection with 0.9% (9 mg\/mL) benzyl alcohol as antimicrobial preservative. No buffer, salt or tonicity agent\u003c\/b\u003e\u003cspan\u003eComposition\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"pp-fi\"\u003e\n\u003cb class=\"pp-v3\"\u003e5.7, range 4.5–7.0 for Bacteriostatic Water for Injection USP\u003c\/b\u003e\u003cspan\u003epH\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"pp-fi\"\u003e\n\u003cb class=\"pp-v2\"\u003eSterile and non-pyrogenic as supplied\u003c\/b\u003e\u003cspan\u003eSterility\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"pp-fi\"\u003e\n\u003cb class=\"pp-v3\"\u003e3ml, 5ml and 10ml vials, rubber-stoppered for repeat entry\u003c\/b\u003e\u003cspan\u003ePresentations\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"pp-fi\"\u003e\n\u003cb class=\"pp-v3\"\u003eRoom temperature, protected from light and freezing\u003c\/b\u003e\u003cspan\u003eStorage\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\u003c\/section\u003e\u003csection class=\"pp-sec\"\u003e\u003cdiv class=\"pp-in\"\u003e\u003cdiv class=\"pp-prose pp-wide\"\u003e\n\u003cp class=\"pp-kick\"\u003eMechanism\u003c\/p\u003e\n\u003ch2\u003eHow it works\u003c\/h2\u003e\n\u003cp\u003eBacteriostatic water is not a peptide. It is a diluent — water for injection with a single additive, benzyl alcohol, at 0.9% by volume, which is 9 mg per millilitre.\u003c\/p\u003e\n\u003cp\u003eThe additive is the entire point. Benzyl alcohol is bacteriostatic rather than bactericidal: it inhibits the multiplication of organisms introduced into the vial rather than sterilising a contaminated solution. It does not rescue a compromised vial; it suppresses growth of the small numbers of organisms a stopper penetration can carry in, so that a vial entered several times over a working period does not become a culture. Its activity is attributed to disruption of microbial membrane integrity.\u003c\/p\u003e\n\u003cp\u003eSterile water for injection contains no preservative. It is sterile at the point of manufacture and remains so only until the stopper is first penetrated, after which it is single-use. For a lyophilised peptide reconstituted once and drawn down repeatedly over days, that is the wrong diluent — which is why bacteriostatic water is the convention for repeat-entry research vials, and sterile water the convention for single-use preparations and for assays where benzyl alcohol would interfere.\u003c\/p\u003e\n\u003cp\u003eTwo further distinctions matter. Bacteriostatic water is not saline: it contains no sodium chloride and is not isotonic. And it is not universally compatible. The Bacteriostatic Water for Injection USP labelling is explicit that benzyl alcohol-containing solutions must not be used in neonates, and that only preservative-free sterile water should be used for that population. The laboratory parallel is assay interference: benzyl alcohol is an organic solvent at low concentration and can affect chromatographic, cell-based and protein-binding assays.\u003c\/p\u003e\n\u003c\/div\u003e\u003c\/div\u003e\u003c\/section\u003e\u003csection class=\"pp-sec pp-alt\"\u003e\u003cdiv class=\"pp-in\"\u003e\n\u003cp class=\"pp-kick\"\u003eEvidence\u003c\/p\u003e\n\u003ch2\u003eKey research facts\u003c\/h2\u003e\n\u003cdiv class=\"pp-cards\"\u003e\n\u003cdiv class=\"pp-card\"\u003e\n\u003cspan class=\"pp-num\"\u003e01\u003c\/span\u003e\u003cp\u003eSterile water for injection containing 0.9% (9 mg\/mL) benzyl alcohol as antimicrobial preservative.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"pp-card\"\u003e\n\u003cspan class=\"pp-num\"\u003e02\u003c\/span\u003e\u003cp\u003eBacteriostatic, not bactericidal — inhibits microbial growth, does not sterilise a contaminated vial.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"pp-card\"\u003e\n\u003cspan class=\"pp-num\"\u003e03\u003c\/span\u003e\u003cp\u003eSterile water for injection is preservative-free and therefore single-use once penetrated.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"pp-card\"\u003e\n\u003cspan class=\"pp-num\"\u003e04\u003c\/span\u003e\u003cp\u003eContains no salt or buffer; not isotonic and not interchangeable with 0.9% sodium chloride.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\u003c\/section\u003e\u003csection class=\"pp-sec\" id=\"prep\"\u003e\u003cdiv class=\"pp-in\"\u003e\n\u003cp class=\"pp-kick\"\u003eHandling\u003c\/p\u003e\n\u003ch2\u003ePreparation \u0026amp; dosage\u003c\/h2\u003e\n\u003cdiv class=\"pp-prose pp-wide\"\u003e\n\u003cp\u003eThe following is general laboratory technique for reconstituting lyophilised research peptides. It is not a protocol for preparing material for administration to humans or animals.\u003c\/p\u003e\n\u003cp\u003eBegin with both vials at ambient temperature. A cold peptide vial condenses atmospheric moisture onto the cake, and moisture is the principal cause of incomplete dissolution.\u003c\/p\u003e\n\u003cp\u003eDecide the volume before piercing anything. Concentration is mass divided by volume: a 10mg peptide vial reconstituted with 2 mL gives 5 mg\/mL; the same vial with 1 mL gives 10 mg\/mL. Choose the volume that puts the working concentration where the model needs it.\u003c\/p\u003e\n\u003cp\u003eSwab both stoppers with 70% isopropyl alcohol and allow them to dry. Alcohol that is still wet is not doing anything.\u003c\/p\u003e\n\u003cp\u003eDraw the intended volume of bacteriostatic water. Insert the needle into the peptide vial at an angle so the stream runs down the inner glass wall. Do not aim it at the lyophilised cake — the mechanical force of a direct stream is a common cause of degradation. Release the diluent slowly.\u003c\/p\u003e\n\u003cp\u003eWithdraw the needle and let the vial stand. Most cakes dissolve within a few minutes. If material remains, roll the vial gently between the palms or swirl it. Do not shake, vortex or invert vigorously; foaming indicates denaturation at the air-liquid interface and is the visible sign of a peptide being damaged.\u003c\/p\u003e\n\u003cp\u003eInspect the solution. It should be clear and free of particulates. Copper-containing peptides such as GHK-Cu are the intended exception, giving a blue to blue-violet solution.\u003c\/p\u003e\n\u003cp\u003eLabel the vial with the compound, concentration and reconstitution date, and store refrigerated at 2–8 °C, protected from light. Aliquot before freezing rather than freeze-thawing repeatedly.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cfigure class=\"pp-fig\"\u003e\u003cimg class=\"pp-img\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0984\/2422\/6090\/files\/purist-reconstitution-steps.png?v=1785967669\" alt=\"Four-step peptide reconstitution guide: bring the vial to room temperature, add bacteriostatic water down the vial wall, roll gently without shaking, and store refrigerated at 2 to 8 degrees Celsius\" width=\"1400\" height=\"900\" loading=\"lazy\" decoding=\"async\"\u003e\u003c\/figure\u003e\n\u003c\/div\u003e\u003c\/section\u003e\u003csection class=\"pp-sec pp-alt\" id=\"faq\"\u003e\u003cdiv class=\"pp-in\"\u003e\n\u003cp class=\"pp-kick\"\u003eQuestions\u003c\/p\u003e\n\u003ch2\u003eFrequently asked questions\u003c\/h2\u003e\n\u003cdiv class=\"pp-faq\"\u003e\n\u003cdetails class=\"pp-ac\"\u003e\u003csummary\u003eWhat is the difference between bacteriostatic water and sterile water for injection?\u003c\/summary\u003e\u003cdiv class=\"pp-ab\"\u003e\u003cp\u003eOne additive. Both are sterile, non-pyrogenic water; bacteriostatic water also contains 0.9% benzyl alcohol as a preservative. That preservative inhibits microbial growth across repeated stopper penetrations, so a vial can be entered more than once. Sterile water for injection has no preservative and is a single-use material once penetrated.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\u003cdetails class=\"pp-ac\"\u003e\u003csummary\u003eHow much bacteriostatic water should be added to a peptide vial?\u003c\/summary\u003e\u003cdiv class=\"pp-ab\"\u003e\u003cp\u003eThere is no universal figure — the volume sets the concentration, and the concentration should suit the research model. Concentration equals the mass in the vial divided by the volume added: a 10mg vial with 2 mL gives 5 mg\/mL. Where a supplier specifies a volume, that specification takes precedence.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\u003cdetails class=\"pp-ac\"\u003e\u003csummary\u003eWhy should the vial not be shaken?\u003c\/summary\u003e\u003cdiv class=\"pp-ab\"\u003e\u003cp\u003eShaking drives peptide molecules to the air-liquid interface, where they unfold and aggregate. Foaming is the visible sign. The result is loss of assayed potency with no change in appearance once the foam settles, which is why the damage goes unnoticed. Swirl or roll instead, and allow time for dissolution.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\u003cdetails class=\"pp-ac\"\u003e\u003csummary\u003eWhich size should be selected, and can one vial serve several peptides?\u003c\/summary\u003e\u003cdiv class=\"pp-ab\"\u003e\u003cp\u003eMatch the vial to the work in hand. A 3ml vial suits a single reconstitution; 5ml and 10ml suit several within the preservative's in-use period. One vial can be entered repeatedly — that is the purpose of the preservative — provided the stopper is swabbed with 70% isopropyl alcohol before every entry and a fresh needle used each time. Never return drawn diluent to the vial.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\u003cdetails class=\"pp-ac\"\u003e\u003csummary\u003eHow is purity verified?\u003c\/summary\u003e\u003cdiv class=\"pp-ab\"\u003e\u003cp\u003eBacteriostatic water is a diluent, not a peptide, and is not subject to the HPLC assay applied to our peptide range. It is supplied to the sterile, non-pyrogenic, 0.9% benzyl alcohol specification described above. Every peptide batch we sell is independently assayed by HPLC at Bioindustrial Services in Boksburg; our testing protocol is set out on our \u003ca href=\"\/pages\/quality-standards\"\u003equality standards page\u003c\/a\u003e.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\u003cdetails class=\"pp-ac\"\u003e\u003csummary\u003eHow long does delivery take?\u003c\/summary\u003e\u003cdiv class=\"pp-ab\"\u003e\u003cp\u003eOrders are dispatched from the Netherlands with tracked courier delivery — 1–3 business days within the Netherlands, 2–5 business days to the rest of the EU. Full timelines are set out on our \u003ca href=\"\/pages\/delivery-process\"\u003edelivery process page\u003c\/a\u003e.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\u003cdetails class=\"pp-ac\"\u003e\u003csummary\u003eWhat is the returns policy?\u003c\/summary\u003e\u003cdiv class=\"pp-ab\"\u003e\u003cp\u003eUnopened vials in original condition may be returned within the stated window; full terms are in our \u003ca href=\"\/pages\/return-refund-policy\"\u003erefund policy\u003c\/a\u003e.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\u003c\/section\u003e\u003csection class=\"pp-links\"\u003e\u003cdiv class=\"pp-in\"\u003e\n\u003cp class=\"pp-kick\"\u003eExplore\u003c\/p\u003e\n\u003cdiv class=\"pp-chips\"\u003e\n\u003ca class=\"pp-chip pp-chip-lead\" href=\"\/collections\/bacteriostatic-water-supplies\"\u003eAlso in Accessories \u0026amp; Supplies\u003c\/a\u003e\u003ca class=\"pp-chip\" href=\"\/products\/bpc-157\"\u003eBPC-157\u003c\/a\u003e\u003ca class=\"pp-chip\" href=\"\/products\/ghk-cu\"\u003eGHK-Cu\u003c\/a\u003e\u003ca class=\"pp-chip\" href=\"\/products\/tb-500\"\u003eTB-500\u003c\/a\u003e\u003ca class=\"pp-chip pp-chip-alt\" href=\"\/pages\/recovery-peptide-stack\"\u003eRecovery Stack\u003c\/a\u003e\u003ca class=\"pp-chip pp-chip-alt\" href=\"\/pages\/peptide-stacks\"\u003eSkin Recovery Stack\u003c\/a\u003e\u003ca class=\"pp-chip pp-chip-alt\" href=\"\/pages\/gh-optimisation-peptide-stack\"\u003eGH Optimisation Stack\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\u003c\/section\u003e\n\u003c\/div\u003e\n\u003cscript type=\"application\/ld+json\"\u003e{\"@context\":\"https:\/\/schema.org\",\"@type\":\"FAQPage\",\"mainEntity\":[{\"@type\":\"Question\",\"name\":\"What is the difference between bacteriostatic water and sterile water for injection?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"One additive. Both are sterile, non-pyrogenic water; bacteriostatic water also contains 0.9% benzyl alcohol as a preservative. That preservative inhibits microbial growth across repeated stopper penetrations, so a vial can be entered more than once. Sterile water for injection has no preservative and is a single-use material once penetrated.\"}},{\"@type\":\"Question\",\"name\":\"How much bacteriostatic water should be added to a peptide vial?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"There is no universal figure. The volume sets the concentration, and the concentration should suit the research model. Concentration equals the mass in the vial divided by the volume added: a 10mg vial with 2 mL gives 5 mg\/mL. Where a supplier specifies a volume, that specification takes precedence.\"}},{\"@type\":\"Question\",\"name\":\"Why should the vial not be shaken?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"Shaking drives peptide molecules to the air-liquid interface, where they unfold and aggregate. Foaming is the visible sign. The result is loss of assayed potency with no change in appearance once the foam settles, which is why the damage goes unnoticed. Swirl or roll instead, and allow time for dissolution.\"}},{\"@type\":\"Question\",\"name\":\"Which size should be selected, and can one vial serve several peptides?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"Match the vial to the work in hand. A 3ml vial suits a single reconstitution; 5ml and 10ml suit several within the preservative's in-use period. One vial can be entered repeatedly, which is the purpose of the preservative, provided the stopper is swabbed with 70% isopropyl alcohol before every entry and a fresh needle used each time. Never return drawn diluent to the vial.\"}},{\"@type\":\"Question\",\"name\":\"How is purity verified?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"Bacteriostatic water is a diluent, not a peptide, and is not subject to the HPLC assay applied to our peptide range. It is supplied to the sterile, non-pyrogenic, 0.9% benzyl alcohol specification. Every peptide batch we sell is independently assayed by HPLC at Bioindustrial Services in Boksburg; our testing protocol is set out on our quality standards page at \/pages\/quality-standards.\"}},{\"@type\":\"Question\",\"name\":\"How long does delivery take?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"Orders are dispatched from the Netherlands with tracked courier delivery — 1–3 business days within the Netherlands, 2–5 business days to the rest of the EU. Full timelines are set out on our delivery process page at \/pages\/delivery-process.\"}},{\"@type\":\"Question\",\"name\":\"What is the returns policy?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"Unopened vials in original condition may be returned within the stated window; full terms are in our refund policy at \/pages\/return-refund-policy.\"}}]}\u003c\/script\u003e","brand":"My Store","offers":[{"title":"3ml","offer_id":49172126564575,"sku":null,"price":7.95,"currency_code":"EUR","in_stock":true},{"title":"5ml","offer_id":51370153115871,"sku":null,"price":8.75,"currency_code":"EUR","in_stock":false},{"title":"10ml","offer_id":51370153148639,"sku":null,"price":10.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0808\/8787\/3759\/files\/BacteriostaticWater3ML_5d1b1693-d1f2-4468-89d1-1dc2a9401290.png?v=1778674585"}],"url":"https:\/\/puristpeptides.nl\/collections\/bacteriostatic-water-supplies.oembed","provider":"Purist Peptides NL","version":"1.0","type":"link"}