GHK-CU

SKIN REJUVENATION • WOUND HEALING • HAIR GROWTH • ANTI-AGING RESEARCH

RECONSTITUTION (MIXING)
• Add 3 ml Bacteriostatic Water to the vial
• Roll gently between your palms until fully dissolved (do not shake)
• Store the reconstituted vial in the refrigerator (2–8 °C)

CONCENTRATION CALCULATION
• 100 mg ÷ 3 ml ≈ 33.3 mg per ml

Insulin Syringe Reference
• Every 10 insulin units (0.1 ml) ≈ 3.3 mg

CORE RESEARCH DOSAGE GUIDELINES

Purpose
• Skin regeneration research
• Tissue repair and wound-healing studies
• Hair follicle stimulation models
• Longevity and anti-aging research

Standard Protocol
• 250 mcg – 3 mg per injection

Frequency
• 1× daily for injectable research protocols
• Topical or microneedling applications may be studied per session

Advanced Protocol
• 1–3 mg daily, depending on research model
• Do not exceed 3 mg per day in most protocols

Administration
• Subcutaneous micro-injection
• Also studied in topical or microneedling delivery systems

Injection Sites
• Rotate injection sites regularly

SYRINGE MEASUREMENT GUIDE

250 mcg — 0.007 ml — <1 unit
500 mcg — 0.015 ml — 1.5 units
1 mg — 0.03 ml — 3 units
2 mg — 0.06 ml — 6 units
3 mg — 0.09 ml — 9 units

Accuracy Note

• For doses under 0.1 ml, 30–50 unit insulin syringes are typically used for higher measurement precision.

1.⁠ ⁠Skin Rejuvenation & Anti-Aging Research
• (Dermal thickness, elasticity, collagen renewal)
• Dose: 250–500 mcg
• Frequency: Once daily or every other day
• Route: Subcutaneous or intradermal
• Duration: 6–10 weeks
• Notes: Low-dose protocols often sufficient for cosmetic endpoints

2.⁠ ⁠Wound Healing & Tissue Repair
• (Post-procedure healing, scar modulation)
• Dose: 1–2 mg
• Frequency: Once daily
• Route: Local subcutaneous or intradermal near target area
• Duration: 4–8 weeks
• Notes: Supports angiogenesis and fibroblast activity

3.⁠ ⁠Hair Growth & Scalp Regeneration
• (Follicular support, vascular supply, hair density)
• Dose: 500 mcg – 1.5 mg
• Frequency: 3–5× weekly
• Route: Intradermal scalp administration
• Duration: 8–12 weeks
• Notes: Avoid excessive daily dosing to limit vascular overgrowth

4.⁠ ⁠Intensive Skin Repair / Post-Laser or Microneedling Research
• (Barrier recovery, collagen induction)
• Dose: 1–3 mg
• Frequency: On treatment days only
• Route: Intradermal or topical application to treated area
• Duration: As per procedural protocol
• Notes: Do not exceed 3 mg/day

5.⁠ ⁠Longevity & Cellular Repair Research
• (Fibroblast signaling, tissue resilience)
• Dose: 250–500 mcg
• Frequency: 3–5× weekly
• Duration: 8–10 weeks
• Notes: Suitable for long-term, low-dose protocols

6.⁠ ⁠Maintenance / Long-Term Skin Support
• Dose: 250 mcg–3mg
• Frequency: Every other day or 2–5× weekly
• Duration: Ongoing after full cycle
• Notes: Minimizes copper load while maintaining benefits

Copper-Related Safety Considerations
Excessive or prolonged exposure above 3 mg/day, especially when combined with high-dose copper supplementation, may increase risk of copper accumulation.

Possible signs of copper overload include:
• Fatigue or nausea
• Metallic taste
• Elevated liver enzymes (AST/ALT)
• Neurological irritability or confusion
• Use caution in research models involving:
• Impaired liver function
• Copper metabolism disorders (e.g. Wilson-type models)

Cycle Duration
GHK-Cu does not require cycling.
It is a naturally occurring copper peptide found in the human body and is generally used continuously rather than in short cycles.

Typical Usage Timeframes
Continuous use: 3–6 months or longer
Many users continue long-term as part of skin, hair, tissue, or recovery support
Can be paused and restarted if needed, but this is not required

Why It Doesn’t Need Cycling
o Naturally present in plasma, saliva, and urine
o Supports cell signaling, tissue repair, collagen synthesis, and inflammation balance
o Does not cause receptor down-regulation like some hormones or stimulatory peptides
o No “on/off” rebound effect when stopped

Key Research Benefits
• Stimulates collagen and extracellular matrix renewal
• Enhances wound healing and tissue regeneration
• Supports hair follicle vascularization and growth
• Improves skin texture, elasticity, and repair capacity
• Activates regenerative and longevity-associated pathways